Antibody purification and aggregation assessment               

Bispecific mAb was purified from the harvested cell culture super- natant by Protein A affinity chromatography using 200 μL volume PhyTip® columns (PhyNexus Inc., San Jose, CA, USA) containing 20 μL of ProPlus (MabSelect SuReTM) affinity resin (PhyNexus Inc, San Jose, CA, USA) with a Tecan Freedom EVO® 200 robotic liquid handler (TECAN Group Ltd, M ̈annedorf, Switzerland).